Pancratium maritimum L. (Sea Daffodil) is a bulbous perennial plant inhabiting sandy dunes along Bulgarian Black Sea coast. Its large scented white flowers are highly attractive and often collected by tourists. Loss of natural populations and habitats led to protection measures: the species is included in the Red Data Book of Bulgaria and is under the regulations of the Biodiversity Act. Mature seeds of P. maritimum collected from the wild were used as initial material for in vitro culture initiation. The seeds were surface sterilized using two-step procedure (Panayotova et al. (in press)). Fast germination on water agar was followed by relatively slow shoot development on solidified MS medium supplemented with BAP and NAA. Growth of the obtained cultures was improved by modification of the auxin and cytokinin levels in the media. Rapid growth and leaf elongation was observed by cultivation of bulblets in liquid MS medium supplemented with 2 mg l^-1 BAP and 0.15 mg l^-1 NAA. Subsequent subcultivation on solidified medium resulted in shoot formation between the scales of the bulblet sectors within 2 weeks. Such promising results give opportunities to develop a rapid propagation method for conservation and reintroduction purposes.