Actively growing shoot tips isolated from an initial in vitro culture of Populus euphatica Oliv. were encapsulated in sodium-alginate matrix. Growth media with different composition were used for cultivation of the alginate beads. High frequencies of both viability and regrowth of explants were registered in all variants tested. It was found that the composition of the regeneration medium significantly affected the regrowth parameters of the explants. The medium based on MS nutrients supplemented with 3% (w/v) sucrose and 1.3 μM BAP provoked formation of shoots with significantly greater length and fresh weight than those at the rest of the variants. In 75.0% of the explants the regrowth event was accompanied by development of new axillary shoots, which additionally increased the proliferation rate and productivity of the culture.