High frequency micropropagation of Arundina graminifolia (D. Don.) Hochr. through protocorm-like bodies (PLBs) using node explants was established. Node explants derived from three-year-old field grown plants were cultured on half-strength MS medium with either 6.97 µM kinetin (Kn), or 15% coconut water (CW) or 13.3 µM BA favoured sprouting of the axillary bud. Subsequent culture of these emerged buds on medium having 44.4 µM BA facilitated formation of PLBs (a mean of 5.4) from the base. Transfer of PLBs to medium with the same level of BA (44.4 µM) PLBs favoured enhanced proliferation. The 6th subculture of PLBs on medium with 44.4 µM BA yielded > 100 PLBs. The PLB proliferation did not exhibit a decline up to 10^th subculture. Conversion of PLBs to shoots or plantlets occurred at high rate (89%) upon transfer to half-strength MS medium containing 6.97 µM Kn. Half-strength MS medium with 1 g l^-1 activated charcoal was effective for rooting, and the rooted plants exhibited 91% survival in field conditions.