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Propagation of Ornamental Plants
7(1): 43-48, 2007
IN VITRO PROPAGATION OF CHIRITA HETEROTRICHA MERR.
Zheng-Hui Tang1, Lei Shi2*, Wei-Lun Chen2, and Hong-Hui Lin1*
1Key laboratory of Bio-resources and Eco-environment (Ministry of Education), College of Life Science,
Sichuan University, Chengdu 610064 Sichuan, P. R. China
2*Institute of Botany, Chinese Academy of Sciences, Beijing 100093, P. R. China
*Fax: +86-10-82593616, *E-mail: shilei67@263.net
Abstract
The present study aimed to establish a system to regenerate an ornamental and endemic species Chirita heterotricha Merr. A procedure for the in vitro plant regeneration from leaf explants of C. heterotricha is described. Culturing small leaf-sections of C. heterotricha on MS medium supplement with 0.1 mg l-1 NAA and 0.1 mg l-1 BA resulted in the highest adventitious buds induction rate (39.5 adventitious buds per explant). Consequently, regenerated plants were established after rooting of shoots on half-strength MS medium with 5 g l-1 activated charcoal, 30 g l-1 sucrose. Rooted plantlets, following acclimatization in a greenhouse, were successfully transferred to field conditions, and 95% of the plants survived. Application of this protocol has potential for mass multiplication of this ornamental species in a limited tim
Key words: Adventitious buds, Chirita heterotricha Merr., in vitro plant regeneration, leaf laminae
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