ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
6(3): 149-155, 2006

EFFICIENT MICROPROPAGATION OF JAPANESE PHOTINIA [PHOTINIA GLABRA (THUNB.) MAXIM.] RETAINING GENETIC AND EPIGENETIC STABILITY

Yidan Li¹, Wanli Guo¹, Xiaoming Liu², Xiaohui Shan¹, Fengxia Li¹, Zhongheng Zhang², and Bao Liu1*

¹Laboratory of Plant Molecular Epigenetics, Institute of Genetics and Cytology, Northeast Normal University, Changchun 130024, China, *Fax: + 86-431-5099822, *E-mail: baoliu6677@yahoo.com.cn ² School of Life Science and Engineering, Qiqihar University, Qiqihar 161006, China


Abstract
Japanese Photinia [Photinia glabra (Thunb.) Maxim.] is a high-valued woody ornamental plant. Although several reports have showed that in vitro micropropagation is a feasible method to produce large quantities of clonal plants from some hybrid species of genus Photinia, no information is available on micropropagation of Japanese Photinia. Here, we report on a tissue culture system for efficient micropropagation of Japanese Photinia by enhanced branching of axillary buds taken from branches of a single donor tree. The culture system consists of choosing the suitable explant coupled with sequential use of three media, namely, the bud-induction medium (MS medium supplemented with 1.2 mg l-1 BAP, 0.2 mg l-1 NAA), subculture medium (WPM medium added with 0.75 mg l-1 BAP, 0.15 mg l-1 NAA) and root-induction medium (half-strength MS medium fortified with 2.5 mg l-1 IAA and 0.3 mg l-1 IBA). In addition, by using AFLP and MSAP markers we investigated the genetic and DNA methylation pattern stability of samples of 36 and 24 morphologically normal plants respectively, which were randomly taken from a population of more than 100,000 micropropagated plants established in the field. We found that of the 615 and 392 reproducible bands scored respectively for AFLP and MSAP, no evidence for occurrence of genetic or epigenetic instability. This, together with phenotypic uniformity of the micropropagated population, suggests that micropropagation through enhanced axillary bud branching ensured genetic and epigenetic fidelity of the donor plant in Japanese Photinia. Therefore, the protocol reported here can be readily employed for large-scale commercial propagation of this ornamental plant species. Japanese Photinia [Photinia glabra (Thunb.) Maxim.] is a high-valued woody ornamental plant. Although several reports have showed that in vitro micropropagation is a feasible method to produce large quantities of clonal plants from some hybrid species of genus Photinia, no information is available on micropropagation of Japanese Photinia. Here, we report on a tissue culture system for efficient micropropagation of Japanese Photinia by enhanced branching of axillary buds taken from branches of a single donor tree. The culture system consists of choosing the suitable explant coupled with sequential use of three media, namely, the bud-induction medium (MS medium supplemented with 1.2 mg l-1 BAP, 0.2 mg l-1 NAA), subculture medium (WPM medium added with 0.75 mg l-1 BAP, 0.15 mg l-1 NAA) and root-induction medium (half-strength MS medium fortified with 2.5 mg l-1 IAA and 0.3 mg l-1 IBA). In addition, by using AFLP and MSAP markers we investigated the genetic and DNA methylation pattern stability of samples of 36 and 24 morphologically normal plants respectively, which were randomly taken from a population of more than 100,000 micropropagated plants established in the field. We found that of the 615 and 392 reproducible bands scored respectively for AFLP and MSAP, no evidence for occurrence of genetic or epigenetic instability. This, together with phenotypic uniformity of the micropropagated population, suggests that micropropagation through enhanced axillary bud branching ensured genetic and epigenetic fidelity of the donor plant in Japanese Photinia. Therefore, the protocol reported here can be readily employed for large-scale commercial propagation of this ornamental plant species.

Key words: AFLP, axillary bud, genetic and epigenetic stability, Japanese Photinia [Photinia glabra (Thunb.) Maxim], micropropagation, MSAP



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