ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
6(3): 140-144, 2006

MASS CLONAL PROPAGATION OF VANDA TERES (ROXB.) LINDLE THROUGH IN VITRO CULTURE OF NODAL SEGMENTS

Mohammad Firoz Alam¹*, Pinaki Sinha¹, and Mohammad Lokman Hakim2

¹Department of Botany, Rajshahi University, Rajshahi, Bangladesh
²Institute of Food and Radiation Biology, Bangladesh Atomic Energy Commission,
Gonokbari, Savar, Dhaka, Bangladesh, *Fax: + 880-721-750064, *E-mail: falambiotech@lycos.com


Abstract
A protocol for continuous high frequency regeneration of Vanda teres through long term-culture of nodal segment derived protocorms was achieved in Vacin and Went (VW) medium. Nodal segments cultured on VW medium with 2.0 mg l-1 Kn + 0.5 mg l-1 NAA + 2 % sucrose + 15% coconut water (CW) + 2 g l-1 peptone produced an average of 25 protocorm like bodies (PLBs) within 4 months. PLBs were multiplied by subculturing on the same nutrient medium, where a PLB multiplied into 10-15 PLBs within 2 months. Shoots from PLBs were achieved by subculturing the PLBs on VW medium supplemented with 2% sucrose + 2 g l-1 peptone + 1 g l-1 active charcoal + 15% CW + 150 mg l-1 L-glutamine within 2 months and by this time new PLBs induced from the base of the developing shoots. Shoots rooted on VW medium supplemented with 2% sucrose, 2 g l-1 peptone, 15% CW, 1 g l-1 active charcoal, 1.0 mg l-1 IBA with or without 50 g l-1 banana pulp. By repeated subculture of PLBs on plantlet regeneration medium, plantlets as well as PLBs were produced continuously. About 8 months were required from PLBs induction to the production of plantlets for transplantation.

Key words: clonal propagation, nodal segment, orchid, protocorm-like bodies (PLBs) formation, Vanda teres





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