Propagation of Ornamental Plants
6(3): 128-133, 2006
PLANT REGENERATION BY SOMATIC EMBRYOGENESIS FROM IMMATURE SEEDS OF STERNBERGIA CANDIDA MATHEW ET T. BAYTOP, AN ENDANGERED ENDEMIC PLANT OF TURKEY
Iskender Parmaksiz¹ and Khalid Mahmood Khawar 2*
¹Department of Biology, Faculty of Science and Arts, Gazi Osman Pasa University, Tasliciftilik, Tokat,Turkey,
2*Institute of Biotechnology, Department of Field Crops, Faculty of Agriculture, University of Ankara, Diskapi, 06110 Ankara, Turkey, *Fax: +90-312-3179815, *E-mail: firstname.lastname@example.org
Tissue culture techniques have been established as a useful approach for in vitro conservation of endemic plant species. This report describes in vitro bulblet production of Sternbergia candida Mathew et T. Baytop, an endangered plant species endemic to the Mugla and Antalya provinces of Turkey, through somatic embryogenesis from immature seeds. The best embryogenesis was observed on MS medium containing 2 mg l-1 6-benzylaminopurine (BAP) and 0.50 mg l-1 Potassium salt of α-naphthalene acetic acid (NAA). The bulblets were converted to plantlets on MS medium with or without 5 g l-1 active charcoal. The addition of active charcoal to the plant conversion media had a complimentary effect and resulted in faster growth and development of roots in the converted plantlets. These were acclimatized in the green house for transfer to the fields to evaluate flowering behavior and the extent of genetic diversity in the morphology of the plants.
Key words: endemic, genetic diversity, immature seeds, in vitro, multiplication, Sternbergia candida