ISSN 1311-9109 Journal Content





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on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
6(3): 121-127, 2006

SOMATIC EMBRYOGENESIS INDUCTION FROM IN VITRO LEAF CULTURES OF LISIANTHUS (EUSTOMA GRANDIFLORUM (RAF.) SHINN.)

Duong Tan Nhut1*, Nguyen Si Tuan², Hua My Ngoc², Phan Nha Uyen¹, Nguyen Trinh Don¹, Nguyen Thanh Mai², and Jaime A. Teixeira da Silva³

¹Da Lat Institute of Biology, 116 Xo Viet Nghe Tinh, Da Lat, Lam Dong, Vietnam,
*Tel.: +84-63-831056, *Fax: +84-63-831028, *E-mail: duongtannhut@gmail.com
²Open University of Ho Chi Minh City, 97 Vo Van Tan, Dist. 3, Ho Chi Minh City, Vietnam
³Faculty of Agriculture, Kagawa University, Miki-cho, Ikenobe, Kagawa-ken, 761-0795, Japan


Abstract
In this paper, somatic embryogenesis induction of Eustoma grandiflorum is presented. Each in vitro-derived leaf was horizontally cut into two segments and cultured on MS (Murashige and Skoog, 1962) medium supplemented with BA, NAA or 2,4-D, individually or in combination. Embryogenic calli formation was observed on the leaf segments cultured on MS medium supplemented with various concentrations of 2,4-D (0.5, 1.0, 2.0, 3.0 or 4.0 mg l-1) in the dark. Embryonic callus formation rate was 85.5% in all leaf segments on MS medium supplemented with 0.5 mg l-1 2,4-D after 30 days of culture. Embryogenic calli were subcultured on MS medium supplemented with 1.0 mg l-1 NAA and BA (0.5, 1.0, 2.0, 3.0, 5.0 mg l-1) to induce embryo-like structures (ELSs or globular embryos). ELSs were obtained at a high frequency from embryogenic calli cultured on MS medium supplemented with 0.5 mg l-1 BA and 1.0 mg l-1 NAA after 15 days of culture. Somatic embryo formation occurred on MS medium supplemented with BA at various concentrations (0.5, 1.0, 2.0, 3.0, 4.0 or 5.0 mg l-1) and 100% somatic embryo-derived plantlets were recovered after the conversion of mature embryos on MS medium containing 1.0 mg l-1 BA. Normal development was observed after 21 days of culture.

Key words: embryogenic callus, embryo-like structures, Eustoma grandiflorum, mature embryo, somatic embryogenesis



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