Propagation of Ornamental Plants
6(3): 114-120, 2006
RAPD DETECT NO SOMACLONAL VARIATION IN CRYOPRESERVED CULTURES OF PINUS ROXBURGHII SARG.
Ravindra Balappa Malabadi* and Kallappa Nataraja
Division of Plant Biotechnology, Department of Botany, Karnatak University, Pavate Nagar, Dharwad-580003, Karnataka state, India, E-mail: MLBD712@rediffmail.com and firstname.lastname@example.org
*Present address: Division of Plant Biotechnology, Finnish Forest Research Institute (METLA), Punkaharju Research Unit, Finlandiantie 18, FIN-58450, Punkaharju, Finland, E-mail: email@example.com
This study highlights the use of RAPD analysis for the assessment of somaclonal variation in the somatic seedlings established from cryopreserved embryogenic cultures. Our results indicated that there was no polymorphism detected with a set of primers tested for each genotype. There was 100% monomorphic banding pattern showing clonal uniformity in all the three genotypes of Pinus roxburghii between parental donor field grown plants (control 1), somatic seedlings from the cultures without cryopreservation (control 2) and somatic seedlings via cryopreserved cultures. The embryogenic system used in this study appears to be suitable for true-to-type clonal propagation of mature P. roxburghii trees.
Key words: cryopreservation, embryogenic tissue, mature tree, RAPD, somatic embryogenesis