Scaevola aemula (family Goodeniaceae, commonly known as fanflower) is a horticulturally important species that is endemic to Australia. Plant regeneration from cell suspensions initiated from leaf- and root-derived calli of S. aemula was studied. Cell suspension cultures were induced in an MS medium supplemented with 0.2 mg l^–1 2,4-D, 0.5 mg l^–1 BAP, and 30 g l^–1 sucrose in the dark. After 6 weeks of suspension maintenance, the cells were transferred onto a solidified callus medium and cultured in the dark. Microcalli that formed after 2 weeks were transferred onto a regeneration medium under controlled light conditions. The subsequent shoot regeneration frequencies were 19.2% and 13.9% for microcalli formed from the cell suspensions initiated from leaf- and root-derived calli, respectively. Somatic embryos were also observed in the cell suspension initiated from leaf-derived calli. Thus, these cell suspension cultures may be used as a regeneration system in breeding programs and developmental studies of Scaevola.