Experiments were conducted on four rose rootstocks, Rosa canina ‘Inermis’, R. indica ‘Major’, R.. manetti, and R. multiflora, to attain an improvement in micropropagation. The following factors were studied for optimal shoot propagation: the type and size of explants to be stimulated to axillary shooting, the concentration of 6-benzylaminopurine (BAP) and CaCl₂, the type of gelling agent, and the effect of anti-ethylene factors. To improve rooting, riboflavin was added to an auxin-containing medium, or shoots were transferred after 5 days from the auxin-containing medium to an auxin-free medium. It was found that, depending on the species, the following factors were beneficial for the quantity and quality of shoots: BAP concentration – different for each of the species, the type of the solidifying compound used, the addition of silver nitrate, the initial length of shoots, and the removal of shoot tips. Improvement in rooting was obtained using vitamin B2 in the auxin-containing medium, or when shoots were transferred after a 5-day incubation period to an auxin-free medium with activated charcoal or to perlite saturated with an auxin-free medium.