Propagation of Ornamental Plants
6(1): 34-38, 2006
SOMATIC EMBRYOGENESIS AND PLANT REGENERATION IN ABELMOSCHUS MANIHOT (L.) MEDICUS
Sung Jin Hwang
Department of Oriental Medicine Materials, Dongshin University, Naju 520-714, South Korea Fax: +82-61-330-3309, E-mail: jimhwang@naver.com
Abstract
Plant regeneration through somatic embryogenesis was achieved from callus-culture-derived hypocotyl explants of Abelmoschus manihot. Somatic embryos developed over the surface of embryogenic calli. Callus cultures were initiated from hypocotyl segments on 1/2 B5 or 1/2 MS medium containing various concentrations of auxins (NAA, 2,4-D) in combination with cytokinins (BA, KIN). The highest percentage of callus formation was 98.7% on 1/2 B5 medium supplemented with 0.5 mg l-1 KIN and 2.0 mg l-1 2,4-D. Somatic embryogenesis was achieved after transfer of embryogenic calli to 1/2 MS medium containing 3% sucrose. The average number of somatic embryos per callus clump was 23 ± 2.1 on 1/2 MS medium after 6 weeks of culture. Scanning electron microscope studies were carried out to observe various developmental stages of somatic embryos. About 87% of the somatic embryos developed into plantlets on 1/2 MS medium containing 3% sucrose after 6 weeks of culture.
Key words: Abelmoschus manihot (L.) Medicus, hypocotyl explants, plant regeneration, somatic embryogenesis
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