Propagation of Ornamental Plants
5(4): 192-198, 2005
SOMATIC EMBRYOGENESIS IN DYCKIA DISTACHYA HASSLER (BROMELIACEAE) – AN ENDANGERED BROMELIAD FROM SOUTH BRAZIL
Marcelo Francisco Pompelli¹*, Denise Fernandes² and Miguel Pedro Guerra³
¹Laboratory of Plant Metabolism, Dept Plant Physiology, Federal University of Viçosa, Viçosa, MG,
36571-000, Brazil,*E-mail: email@example.com, *Fax +55 31 3899 2580
²Laboratory of Botanic, West University of Santa Catarina, Videira, SC, 89560-000, Brazil
³Laboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina, 88.034-001, Florianópolis, Santa Catarina, Brazil
Dyckia distachya Hassler is an endemic bromeliad from the western part of the Santa Catarina state, southern Brazil. This species is currently threatened by extinction, since its original habitat has been severely affected by the construction of hydroelectric plants. Micropropagation techniques are efficient tools for the mass propagation and conservation of elite genotypes. In the present work, we studied the factors associated with the induction of somatic embryogenesis in D. distachya. Seeds were employed as an explant source and showed distinct morphogenic responses. Somatic embryogenesis and the regeneration of complete plants were obtained when seeds were inoculated in MS culture medium supplemented with Morel vitamins, Picloran (5-10 µM) and in the presence or absence of 1 µM N6-Benzyladenine (BA) or Kinetin (Kin).
Key words: Atlantic Forest, Bromeliaceae, conservation, somatic embryogenesis.