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Propagation of Ornamental Plants
5(2): 89-94, 2005
TRANSFORMATION OFDENDROBIUM PHALAENOPSIS FITZ. AND D. NOBILE LINDL. WITH CHS AND AP1 GENES
Xiaorong Nie¹², Shuzhen Men¹, Lixin An¹, Meng Li¹, Chunhong Wei¹, Qibing Chen² and Yi Li¹*
¹* Peking-Yale Joint Center for Plant Molecular Genetics and Agrobiotechnology,
The National Laboratory of Protein Engineering and Plant Genetic Engineering,
Department of Biotechnology, College of Life Sciences, Peking University,
Beijing 100871, China, *Fax: 86-10-62754427, *E-mail: liyi@pku.edu.cn
² College of Forestry, Sichuan Agricultural University,
Yaan, Sichuan 625014, China
Abstract
To alter the flower color and flowering time of ornamental orchids Dendrobium phalaenopsis and D. nobile, we transformed the orchids of Dendrobium phalaenopsis and D. nobile with the CHSA gene and the floral meristem identity gene AP1 via Agrobacterium-mediated transformation of protocorm-like bodies (PLBs), respectively. The CHSA gene was cloned from Petunia hybrid, and the AP1 gene was cloned from Arabidopsis. A total of 69 putative transformed lines of D. nobile with the AP1 gene were obtained, and 7 lines had developed roots. While for the CHSA gene, four independent transgenic lines of Dendrobium phalaenopsis were obtained, and three lines had developed roots. PCR analysis detected the CHSA fragment in all the 4 putative transgenic lines, while in untransformed control plants no specific products were detected. Similarly Southern blot analysis of transformants with the AP1 gene showed that putative transgenic lines have hybridization signals, while no hybridization signal for AP1 gene was detected in untransformed control plants.
Key words: Agrobacterium tumefaciens, AP1 gene, CHS gene, flower color, flowering time, protocorm-like bodies, transgenic
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