ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(1): 59-66, 2005

MICROPROPAGATION THROUGH MULTIPLE ADVENTITIOUS SHOOT DIFFERENTIATION FROM CALLUS CULTURES DERIVED FROM MATURE EMBRYOS OF CHRISTMAS TREE SPECIES

Wei Tang* and Ronald J. Newton

East Carolina University, Department of Biology, Howell Science Complex, Greenville, NC 27858, USA, *Tel: (252)-328-2021, *Fax: (252)-328-4178, e-mail: tangw@mail.ecu.edu


Abstract
Micropropagation of Christmas tree species Fraser fir [Abies fraseri (Pursh) Poir], Nordmann fir (Abies nordmanniana Lk.), Virginia pine (Pinus virginiana Mill.), and white pine (Pinus strobus L.) was con­ducted on media DCR, B5, LP, MSG, MS, SH, and TE. Callus was induced from mature embryos of above four species on seven basic media supplemented with 36.2 µM dichlorophenoxyacetic acid (2,4-D), 17.8 µM 6-benzyladenine (BA), and 18.6 µM kinetin. Multiple adventitious shoot formation was obtained from callus cultures of Fraser fir, Nordmann fir, Virginia pine, and white pine on SH and TE medium containing indole-3-acetic acid (IAA) and BA. Regenerated plantlets were produced by transferring adventitious shoots onto TE medium supplemented with 0. 01 µM α-naphthaleneacetic acid (NAA). These results suggested that efficient plant regeneration protocols for micropropagation of Virginia pine and white pine had been developed. However, regeneration protocol for Fraser fir and Nordmann fir are applicable only partially and no regenerated plants have survived in soil. Further improvements in regeneration of Fraser fir and Nordmann fir are needed. Our results could be useful for the future studies on genetic transformation and engineering breeding of pine species..

Key words: Abies fraseri (Pursh) Poir, Abies nordmanniana Lk., Adventitious shoot formation, Christmas tree, micropropagation, Pinus strobus L., Pinus virginiana Mill., plant regeneration



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