A new culture system, the nylon culture system with some advantages of ventilating and low cost (0.003-0.005 USD/bag) was used to propagate Sinningia spp. Plantlets cultured in the nylon system grew better than those in the glass system. A more than 3-fold stem height and number of leaves were obtained in nylon system (Polyethylene, NS) as compared to the glass bottle system. Moreover, the fresh weight of plantlets derived from NS was more than 4-fold higher than control plants in the glass system. Interestingly, in the nylon system when the sucrose concentration was decreased to zero (photoautotrophic growth), the plants manifested good growth ex vitro. After 7 or 14 weeks in the greenhouse, the stem height, leaf number, fresh weight and the diameter of tubers of plants derived from sucrose-free medium were better than those from sucrose-containing medium.