ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(1): 45-50, 2005

THE APPLICATION OF A BAG CULTURE SYSTEM FOR THE IN VITRO PROPAGATION OF SINNINGIA SPP.

Duong Tan Nhut¹*, Nguyen Hong Vu¹, Le Thi Thanh Xuan¹, Tran Thi Ngoc Huong¹, Nguyen Hong Viet¹, Phan Xuan Huyen¹ and Jaime A. Teixeira da Silva²

¹Dalat Institute of Biology, 116 Xo Viet Nghe Tinh, Dalat, Lam Dong, Vietnam *Fax: 84-63-831028, *e-mail: duongtannhut@yahoo.com
²Faculty of Agriculture, Kagawa University, 761-0795 Miki-cho, Kagawa, Japan


Abstract
A new culture system, the nylon culture system with some advantages of ventilating and low cost (0.003-0.005 USD/bag) was used to propagate Sinningia spp. Plantlets cultured in the nylon system grew better than those in the glass system. A more than 3-fold stem height and number of leaves were obtained in nylon system (Polyethylene, NS) as compared to the glass bottle system. Moreover, the fresh weight of plantlets derived from NS was more than 4-fold higher than control plants in the glass system. Interestingly, in the nylon system when the sucrose concentration was decreased to zero (photoautotrophic growth), the plants manifested good growth ex vitro. After 7 or 14 weeks in the greenhouse, the stem height, leaf number, fresh weight and the diameter of tubers of plants derived from sucrose-free medium were better than those from sucrose-containing medium.

Key words: film culture system, nylon (polyethylene) culture system, Sinningia spp.



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