ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(1): 35-44, 2005

THE EFFECT OF TEMPERATURE ON CONVERSION OF WHITE SPRUCE SOMATIC EMBRYOS

Sharon E. Pond

Canadian Forest Service- Maritimes Region, P.O. Box 4000, Fredericton, New Brunswick, Canada E3B 5P7, Fax: + 1 (506) 452 3525, *e-mail: spond@nrcan.gc.ca


Abstract
Germinant quality can be manipulated simply by controlling the temperature at which the embryos are matured and by the timing and duration of the temperature treatment. Plates of maturing white spruce somatic embryos at four stages of development (I- mammilate, II- early cotyledonary, III- cotyledonary and IV- mature) were put in darkened incubators at five temperatures (1, 5, 10, 20, or 30°C) for a period of 0, 1, 2, 4, or 8 weeks. The embryos were then germinated and germinant quality was correlated with the temperature treatment. Conversion was improved by exposing plates of fully mature embryos to 5°C, or immature or mature embryos to 10°C, for a minimum of 4 weeks. Embryos grown at temperatures of 10°C and above can become vitreous or germinate precociously. To preclude this loss in quality, embryos may be stored short-term at 5°C. Stage III & IV embryos were kept for 8 weeks at a temperature of 5°C without any loss of germinant quality.

Key words: Picea glauca (Moench.) Voss, white spruce, cold acclimation, conversion, germination, embryo development, somatic embryogenesis.



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