ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
5(1): 14-18, 2005

WATER RETENTION CAPACITY OF TISSUE-CULTURED PLANTS: PERFORMANCE OF LEAVES FROM IN VITRO GERMINATED MUNGBEAN SEEDLINGS

Geert-Jan de Klerk and Ferry Wijnhoven

Biodiversity and Breeding, Plant Research International, PO Box 16, 6700 AA Wageningen, The Netherlands, Fax: +31317418094, e-mail: geertjan.deklerk@wur.nl


Abstract
Leaves rapidly close their stomata after detachment resulting in a strong reduction of water loss. It has been reported that detached leaves of in vitro produced plants show continuous water loss indicating that they are unable to close the stomata properly and/or that their cuticle is malfunctioning. We examined the water retention capacity (WRC) of detached primary leaves of in vitro germinating seedlings of Vigna radiata (mungbean). It was shown that the poor WRC was most likely due to deterioration of the stomata by the high relative humidity in the headspace. Other features of the headspace, viz., O2, CO2 and ethylene levels, did not play a significant role. Some medium additions improved the WRC somewhat, viz., paclobutrazol and abscisic acid. Other additions such as the ethylene precursor 1-aminocyclopropane-1-carboxylic acid, the ethylene blocker silverthiosulphate and cytokinin had no or a slight negative effect.

Key words: acclimatization, micropropagation, stomata, water retention capacity



copyright (c) 2009, Sejani Publisher. All rights reserved.