Gypsophyla paniculata L. shoots were cultured in aerated and non-aerated vessels. Ventilated vessels had their caps perforated with a 5 mm drill, and the holes were sealed with microporous filters (Milliseal®). Shoots were cultured in vessels with either a) one or two aeration holes, b) in non-aerated Magenta Vessels (MV) or c) non- aerated Jam Vessels (JV) for one month and then transferred to a greenhouse. The in vitro and ex vitro growth of Gypsophyla paniculata plantlets was observed and recorded. Fresh weight and plant height of Gypsophyla plantlets cultured in aerated vessel systems increased significantly and their subsequent growth and development were improved considerably in the greenhouse. Among these systems, two aerated holes with MV and one aerated hole with JV are best suited for Gypsophyla micropropagation and subsequent growth as compared to the other systems.