To overcome various disadvantages of conventional culture vessels and to employ photoautotrophic micropropagation techniques, Tanaka et al. (1996) first developed a practical film culture system the "Miracle Pack" (MP), made of fluorocarbon polymer film (Neoflon® PFA film) and supported by a polycarbonate frame. The PFA film has superior thermal stability, high light transmittance and high gas permeability. While the MP system is more suitable for the micropropagation of various plant species compared to conventional culture vessels, its high cost is a disadvantage. In order to reduce the cost of film culture vessels, a multiple-layered and very inexpensive OTP® film made of TPX (4-methyl-1-pentane polymer) and CPP (a polypropylene) with characteristics similar to the PFA film, has been used to make a novel disposable film culture vessel, termed the "Vitron". In this study, Epidendrum(E. Rouge Magic x E. Joseph Lii 'Mother's Day Koto') shoots at the three leaf stage were cultured under high CO₂ conditions at a low photosynthetic photon flux density in the three film culture systems: MP using PFA film, MP using OTP film and Vitron, with sugar-free liquid modified Vacin and Went medium and rockwool multiblock™ as a substrate. The in vitro and ex vitro growth of Epidendrum plantlets cultured in the three film culture systems were equivalent, producing normal and vigorous plantlets. The net photosynthetic rate of in vitro Epidendrum plantlets cultured in the three film culture systems was also equivalent. This study suggests that the novel Vitron film culture system can replace the conventional culture vessels for the photoautotrophic micropropagation of Epidendrum.