Stem development of shoot cultures from Amelanchier lamarckii on multiplication medium was investigated concerning shoot length, leaf number, stem diameter and xylem development after 4 to 7 weeks. Shoot length was classified in five different shoot length classes. Shoot length, leaf number, stem diameter as well as formation of secondary xylem increased from week 4 to week 7 on multiplication medium. For A. lamarckii each shoot length class was characterized by a certain leaf number and shoot diameter. Leaf number and shoot diameter has hardly changed in one shoot length class in dependence of subculture duration. From this point of view the shoot length can be used to classify different shoot qualities. The development of xylem, however, was dependent on the shoot length class and the subculture period, too. The root formation in shoots with a length from 5 to 30 mm was not different. In shoots smaller than 5 mm or longer than 30 mm, however, the root formation was significantly lower.