Chrysanthemum, Dendranthema x grandiflora (Ramat.) Kitamura, is globally one of the most important floricultural (cut-flower) and ornamental (pot and garden) crops. The last 35 years have seen a tremendous emphasis on its in vitro tissue culture and micropropagation. Due to limitations in transgenic technology of this ornamental plant, research pertaining to its regeneration continues to be intense, focusing on improvement of regeneration in the context of transformation inefficiencies. This review highlights all the available literature that exists on chrysanthemum in vitro culture, and all the pertinent results derived from these regeneration studies, which will serve to assist future investigations into regeneration, the fundamental basic step for successful transformation. Moreover, techniques for chrysanthemum germplasm long-term preservation are an important supplement to tissue culture.