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Propagation of Ornamental Plants
2(1): 45-48, 2002
IN VITRO PRODUCTION OF PATHOGEN-FREE PLANT MATERIAL IN PRUNUS DOMESTICA L.
Alena Gajdosová, Martin Vican, Eva Fábiková, Mariá Gabriela Ostrolucká,
Gabriela Libiaková
Institute of Plant Genetics and Biotechnology Slovak Academy of Sciences, Akademická 2, P.O. Box 39A, 950 07 Nitra, Slovak Republic, Fax: + 421 37 73 366 60,
e-mail: alena.gajdosova@savba.sk
Abstract
An effective regeneration/multiplication system in selected cultivars of Prunus domestica was developed. The best culture medium for regeneration of meristems isolated from mature trees was 1/WPM supplemented with GA3 (0.1 mg l-1), BAP (1 mg l-1) and 2,4-D (0.002 mg l-1) inducing vital shoots with vigorous growth. The highest multiplication rate was achieved on 3/MS multiplication medium supplemented with GA3 (0.1 mg l-1), BAP (1 mg l-1) and IBA (0.1 mg l-1). In vitro propagation of Prunus domestica in combination with chemotherapy enables elimination of pathogens from plant tissue and production of pathogen-free plants.
Key words: Prunus domestica, micropropagation, meristem cultures, chemotherapy
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