Ebenus cretica L. is a potent new flower crop and its vegetative propagation by cuttings is of great interest. Peroxidase activity has been correlated with the rooting ability of cuttings in many plants species. Thus, terminal semi-hardwood cuttings, taken from stock pot plants grown in a greenhouse, were used for studying the peroxidase activity. The effects of PVPP, ascorbic acid and ionic potency were estimated in the extraction buffer. The peroxidase activity in the shoot, bark and xylem was measured at pH 4.5-8.5 in 0.1 M phosphate buffer. The peroxidase patterns, anionic and cationic, were studied with electrophoresis in acrylamide gels. The enzyme activity increased by 50 % in the presence of PVPP. The phosphate buffer of 0.2 M at pH 6.5 showed 1.5 times greater enzyme activity than that of 0.05 M. The protein contents in the shoot, bark and xylem were 10, 13 and 4 µg ml^-1, respectively. In a pH range from 4.5 to 8.5 the enzyme activity had two peaks: one at pH 5.5 and another at pH 7.0. Two or three anionic and one cationic peroxidase patterns were noticed in acrylamide gels.