Ilex aquifolium is an evergreen native plant of Greece of great interest for ornamental use. Efforts for mass propagation aim at the conservation of genetic resources and the enrichment with species of high ornamental value adapted to the environmental conditions of Greece. Selected female and male plants can also be used in plantations for ensuring supply of Christmas branches. Studies were conducted to investigate the effect of: IBA application, rooting system (fog or mist), rooting substrate, supplementary lighting and shading upon rooting of Ilex aquifolium stem cuttings. Cuttings were collected from mature female plants, grown under natural conditions. They were planted in perlite and placed for rooting in fog after the application of 0, 2, 4, 8 or 16 g l^-1 IBA. Treatments with IBA had a strong influence on the percentage rooting, number and length of roots with best results at 4 or 8 g l^-1. In another experiment, rooting of cuttings took place in fog or mist in perlite or a mixture of perlite and peat (2:1). The best rooting (60%) was observed in cuttings planted in perlite and rooted under fog. Rooting was also assessed with or without supplementary lighting during winter or shading during summer. Supplementary lighting had a positive effect on rooting capacity, while shading had a negative effect.