ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
1(1): 25-27, 2001

PROPAGATION OF EBENUS CRETICA L. BY TISSUE CULTURE

Stefanos Hatzilazarou, Athanasios Economou, Theodora Antoniou and
Parthenopi Ralli

Aristotle University, Department of Horticulture, 54006 Thessaloniki, Greece,
e-mail: hatzilaz@agro.auth.gr


Abstract
Propagation of the native plant Ebenus cretica L., via organogenesis was studied. After surface sterilization seeds were germinated in absorbent paper to produce plant material for the in vitro cultures. The explants taken from the seedlings were either epicotyls or shoot-tips. The medium used was MS supplemented with 2 µM of IAA and BA at various concentrations. Also, full- and 1/2-strength in mineral salts of MS were used with 2 µM IAA and 5 µM BA. Microshoots excised from proliferated shoot-tip explants were used as microcuttings for rooting in a hormone-free MS medium with full-, 1/2- and 1/4-strength in mineral salts. Although high percentage of epicotyls of Ebenus cretica responded and formed shoots, the best result was obtained with 5 µM BA. Full-strength in mineral salts of MS medium favoured formation of shoots in comparison to 1/2-strength. Shoot-tip explants from young seedlings responded in vitro producing 3-5 new shoots per explant on average with 10-20 µM BA and 2 µM IAA. Rooting of microcuttings varied according to the strength in mineral salts of MS medium, with the highest rooting percentage obtained in 1/4-strength.

Key words: Ebenus cretica, in vitro culture, micropropagation, new flower crop, pot plant.



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