The study aimed to evaluate the effect of silver (Ag) nanoparticles (NPs) on the surface disinfection of Ardisia mamillata nodal explants and to examine the effect of plant growth regulators on axillary shoot formation and their rooting. Ag NPs at varying concentrations (25-200 mg l^-1) and exposure times (15-60 min) were studied. The most effective surface disinfection treatment was 100 mg l^-1 Ag NPs for 45 min. Nodal explants were cultured on different concentrations (0.0-16.0 µM) of N⁶-benzyladenine (BA), 6-furfurylaminopurine (kinetin, KIN), or 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea) (thidiazuron, TDZ) for axillary shoot induction. The highest axillary shoot induction (96.6%) and maximum number of axillary shoots (4.9) with a length of 3.7 cm were obtained on Murashige and Skoog (MS) medium with 8.0 µM of KIN. Regenerated shoots were cultured on different concentrations (0.0-8.0 µM) of indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), or α-naphthaleneacetic acid (NAA) for rooting. Of the auxins studied, IBA resulted in 100% root induction, with a maximum of 15.2 roots at 2 µM.