The study aimed to develop a protocol for mass propagating Spathiphyllum wallisii ‘Domino’ using rhizome explants. The objective was to investigate the effect of plant growth regulators on adventitious shoot induction, somatic embryogenesis, and in vitro rooting of S. wallisii ‘Domino’. The discs prepared from disinfected rhizomes were placed on a medium with 0.0, 2.0, 4.0, 8.0, or 12.0 μM of N⁶-benzyladenine (BA), 1-phenyl-3-(1,2,3-thiadiazol-5-yl)urea (TDZ) alone or combined with 5.0-30.0 μM of 2,4-dichlorophenoxyacetic acid (2,4-D). The type and concentration of cytokinins significantly affected the induction of adventitious shoots. TDZ, especially at a concentration of 4.0 µM, was found to be more effective than BA in promoting direct adventitious shoot formation. The combination of 2,4-D with either BA or TDZ significantly improved shoot induction. However, higher concentrations of 2,4-D inhibited shoot formation but favored somatic embryogenesis. The combination of 4.0 μM TDZ with 10.0 and 20.0 μM 2,4-D was the most effective in promoting both shoot induction and somatic embryogenesis, respectively. A concentration of 5.0 µM indole-3-butyric acid was found to be the most effective for promoting optimal rooting and growth of adventitious shoots. The highest somatic embryo conversion (93.8%) was achieved at 2.0 µM BA, along with 6.0 roots, a root length of 6.2 cm, and the greatest shoot length (5.3 cm). Plantlets developed on this medium also exhibited the highest fresh weight of 0.547 g.