ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
23(1): 13-21, 2023


Vu Thi Lan1*, Hoang Thi Thu Yen1, Do Tien Phat2, and Nguyen Van Phuong3*

1 Thai Nguyen University of Sciences, Tan Thinh Ward, 250000 Thai Nguyen City, Thai Nguyen Province, Vietnam, *E-mail:
2 Department of Plant Cell Biotechnology, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet str., Cau Giay, 122000 Hanoi, Vietnam
3 University of Science and Technology of Hanoi, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet str., Cau Giay, 122000 Hanoi, Vietnam

Paphiopedilum concolor (Bateman) Pfitzer is a native slipper orchid of Northern Vietnam. Currently, this species has been almost extinct in the wild due to deforestation and overexploitation, and therefore, it is very important to develop an efficient in vitro culture method for conservation. This paper reports the factors affecting high frequency germination and regeneration in vitro of Paphiopedilum concolor, including seed maturity, basal nutrient medium, plant growth regulators and additive organic compounds. The seeds at the age of 190 days after pollination (DAP) showed the highest germination rate (85.0%) and the germination time was 40 days. The germination rates on both half-strength MS and half-strength VW media reached 73.8% and 72.5%, respectively. The optimal medium for protocorm-derived shoot regeneration and growth of planlets was half-strength MS medium supplemented with 1.0 mg l-1 BAP, 0.5 mg l-1 NAA, 100 ml l-1 coconut water, 40 g l-1 potato homogenate, and 30 g l-1 banana homogenate. On this medium the average number of shoots and leaves was 3.3 and 3.6, respectively. The leaves were thick, large, and dark green. Their length reached 1.5 cm. The best rooting effect was observed on the half-strength VW medium supplemented with 1.0 mg l-1 NAA combined with 0.5 mg l-1 AC, and 30 g l-1 banana homogenate. The average number of roots reached 4.5, increasing 2.5 roots compared to the control.

Key words: asymbiotic seed germination, floricultural orchid, in vitro propagation, vulnerable species

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