ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
20(1): 3-11, 2020

HIGHLY EFFECTIVE MICROPROPAGATION AND INDUCTION OF EARLY FLOWERING BY GIBBERELLIC ACID IN PAPHIOPEDILUM DELENATII GUILLAUMIN

Vu Quoc Luan1, Le Kim Cuong1, Hoang Thanh Tung1, Vu Thi Hien1, Do Manh Cuong1, Phan Le Ha Nguyen1, Bui Van The Vinh2, and Duong Tan Nhut1*

1 Tay Nguyen Institute for Scientific Research, Vietnam Academy of Science and Technology, Dalat, Vietnam, *E-mail: duongtannhut@gmail.com
2 Ho Chi Minh City University of Technology (HUTECH), Ho Chi Minh, Vietnam



The micropropagation of Paphiopedilum delenatii via gibberellic acid (GA3) treatment is reported. The juvenile axillary shoots of P. delenatii were isolated and cultured on Schenk and Hildebrandt (SH) medium supplemented with 5 concentrations of GA3 (1.0-5.0 mg l-1) for shoot elongation. Nodal explants were isolated from elongated shoots and placed on medium supplemented with N6-benzyladenine (BA), Kinetin (KIN), or Thidiazuron (TDZ) for shoot multiplication. The highest number of axillary shoots was recorded in SH medium supplemented with 0.5 mg l-1 TDZ after 60 days of culture. Vigorous and uniform plantlets with well-develop shoots and roots were planted in the greenhouse. The 3-year-old P. delenatii plants were treated with GA3 (50-500 mg l-1) for 4 consecutive months to stimulate inflorescence emergence. Moreover, GA3 also contributed positively to the early blooming of P. delenatii plants.

Key words: early flowering, gibberellic acid, in vitro, Paphiopedilum delenatii, shoot elongation



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