Micropropagation of selected Ptilotus clones was investigated. Shoot multiplication was possible on a modified Murashige and Skoog medium (half-strength macroelements) with 2.2 or 4.4 µM BAP and 2.85 µM IAA with up to 3.4 shoots per explant in P. exaltatus and 1.8 shoots per explant in P. nobilis. Rooting in five P. exaltatus clones was induced on a modified MS medium (third-strength macroelements) with 2.5 µM IBA. Depending on the clone the percentage of rooted shoots ranged from 33.6 to 100%. Rooting of clone V could be enhanced by the application of higher IBA concentration (10 µM) from 42.8 to 72.6%. In clone IV by reducing symptoms of hyperhydricity, rooting increased from 32.5 to 82.3%. This improved shoot quality was attained by a subculture on last multiplication medium with reduced BAP concentration (0.88 µM BAP) and an additional subculture on plant growth regulators-free medium. By this treatment the survival after acclimatization increased from 5.0 to 72.7% in clone IV.