The use of light emitting diodes (LEDs) is important in commercial micropropagation of plants due to the economic and biological benefits they confer relative to lighting systems with fluorescent lamps. The aim of this study was to evaluate the effect of LEDs on in vitro shoot multiplication and growth of Anthurium andreanum. For shoot multiplication, nodal segments of the Rosa cultivar were used as explants and grown in Murashige and Skoog culture medium supplemented with 2 mg l^-1 of 6-benzylaminopurine (BAP). To evaluate in vitro rooting and growth, one-cm-long adventitious shoots were cultured in Murashige and Skoog medium without growth regulators. All explants were exposed to lighting with fluorescent lamps (545-610 nm), white LEDs (460 and 560 nm), red LEDs (660 nm), blue LEDs (460 nm), and the combination of blue + red LEDs (460 and 660 nm) at the same photosynthetic photon flux density (25 µmol m^-2 s^-1). After 60 days of culture, the number and length of adventitious shoots were determined. For rooting and shoot growth, the length, number of leaves, rooted shoots, number of roots, and chlorophyll content were evaluated on the sixtieth day. The results showed significant differences during in vitro shoot multiplication and growth. The highest number of adventitious shoots was obtained in blue + red LEDs, while the greatest growth and chlorophyll content was obtained in blue LEDs. These results suggest that LEDs use could be an option for improving shoot multiplication and growth of A. andraeanum plantlets in vitro.