ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
15(3): 89-94, 2015

A NOVEL ROOTING MATRIX AND VESSEL SYSTEM RESULTED IN LARGER PLANTS AND FASTER GROWTH DURING GREENHOUSE ACCLIMATIZATION OF HYDRANGEA QUERCIFOLIA ‘SIKES DWARF’

Jeffrey Adelberg1*, Jacqueline Naylor Adelberg1, and Vijay Rapaka2

1 Departrment of Agricultural and Environmental Science, Clemson University,
Clemson SC, USA 29634 *Fax: + 1-864-656-4960, *E-mail: jadlbrg@clemson.edu
2 Smithers-Oasis Company, 919 Marvin Street, Kent OH, USA 44240



Hydrangea quercifolia ‘Sikes Dwarf’ were grown in agar and the Oasis® foam (InVitro Express -IVE) for 6 weeks in vitro and placed in varied ventilation and acclimatization treatments. Following six weeks in vessels, plants from all treatments rooted well (91%). Shoots from agar and the Oasis® IVE that remained in the laboratory were a little taller than shoots that received pre-transplant bright-light acclimatization in the greenhouse while remaining sterile in flexible film tissue culture vessels. Plants were then transferred to a peat-based soilless media and subsequent greenhouse growth was measured. Although the agar grown plants had started out taller they did not gain height in the greenhouse, while the IVE rooted plants that were acclimatized in the lab increased 1.5- to 1.7-fold and the pre-transplant, bright light acclimatized doubled in height. Leaf size of plants from agar doubled in the greenhouse, whereas the IVE rooted plants increased 3-fold, or more depending on the acclimatization treatment. Hydrangea plants with the largest leaves and best canopies came from treatments that were rooted in IVE and brought through the pre-transplant, bright light, acclimatization while in the flexible film vessels. Improved water, oxygen, and nutrient availability during the in vitro rooting, minimal damage or disturbance to the root system upon transfer, and the pre-hardened shoots allowed for superior greenhouse growth.

Key words: flexible film, liquid culture, microporous membrane, micropropagation, Oasis® InVitro Express, ventilation



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