ISSN 1311-9109 Journal Content

International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy

Propagation of Ornamental Plants
15(3): 113-122, 2015


Fatiha Lebtahi1,2*, Mohamed Brahim Errahmani3, and Nadia Bouguedoura1

1 University of Sciences and Technology Houari Boumediene, Faculty of Biological Sciences, BP 32, Bab Ezzouar, 16111 Algiers, Algeria, *Fax: + 213-21-63-91-41,
2 National Institute of Forestry Research, Laboratory in vitro culture, BP 37,
16048 Chéraga, Algeria
3 Blida 1 University, Faculty of Sciences, Department of Chemistry, BP 270,
route de Soumâa, 09000 Blida, Algeria

Along with Mediterranean forests, Algerian Cork oak forests are undergoing continuous decline and deterioration from year to year. The expansion of Cork oak stands (Quercus suber L.), which are currently populated by old and abandoned trees, demand the availability of thousands of seedlings, which cannot be achieved by traditional methods of propagation. It thus becomes necessary to resort to in vitro culture techniques. Hence it is essential and even a priority to master a standard method of in vitro regeneration with a view to attaining mass production. The aim of this study was to develop a rapid and intense propagation technique of Cork oak. The work reported basically concerns the micropropagation of this species by axillary shoot formation and somatic embryogenesis. Half-strength Murashige and Skoog (1962) medium (MS) was used for axillary shoot induction.The effect of a concentration range of 6-benzylaminopurine (BAP) was tested on the response of the nodes. The combinations of indole-3-butyric acid (IBA) and α-naphtaleneacetic acid (NAA) were studied in the stage of rooting. Concentration 0.6 mg l-1 of BAP has been proved to be the best one. This method of propagation enabled us to achieve a mean multiplication rate of 2.8 ± 0.2 shoots. The combination of IBA and NAA (0.5 mg l-1 IBA + 0.2 mg l-1 NAA) was the best with the highest rooting rate (73.3%). Somatic embryogenesis was induced from mature zygotic embryos on MS medium supplemented with IBA and BAP. The best result was observed with 2 mg l-1 IBA and 2 mg l-1 BAP which not only induced the development of somatic embryos but also permitted their differentiation. Germination and conversion of somatic embryos thus obtained was achieved on MS medium without growth regulators.

Key words: culture media, histology, plant growth regulators, woody species

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