ISSN 1311-9109 Journal Content





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International Symposium
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April 19-24, 2015,
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Propagation of Ornamental Plants
15(3): 101-106, 2015

SOMATIC EMBRYOGENESIS AND PLANT REGENERATION OF
TIGRIDIA PAVONIA (L.F.) DC., A MEXICAN NATIVE ORNAMENTAL PLANT

Amaury Martín Arzate-Fernández1, Hugo González-González1, Luis Miguel Vázquez-García2, Thomas Héctor Norman-Mondragón1, Claudia González-Villarreal3, Stevens Michael Brumbley3, and José Luis Piña-Escutia1*

1 Faculty of Agricultural Science, University Autonomous of State of Mexico, Toluca-Ixtlahuaca Highway km 11.5, University Campus “El Cerrillo”, 50200 Toluca, State of Mexico, Mexico, *Fax: + 52-722-296-55-18, *E-mail: jlpinae@uaemex.mx
2 University Center Tenancingo, University Autonomous of State of Mexico, Tenancingo - Villa Guerrero Highway k.m. 1.5, 52400, Tenancingo, State of Mexico, Mexico
3 University of North Texas, Department of Biological Sciences, W Sycamore, Life Sciences Complex, Denton TX 76203-5017, USA



Tigridia pavonia (L.f.) DC., also known as “tiger flower” is reproduced by seed or by bulb. However, when it is propagated by seed the germination rates are very low and in order to get the mature bulbs it takes at least three years. In the present study, somatic embryos formation was induced through embryogenic callus cultivated on half-strenth Litvay et al. medium supplemented with 0.5 g l-1 L-glutamine and 1 mg l-1 abscisic acid enhanced maturation of somatic embryos. The histological analysis confirmed the unicellular origin of somatic embryos showing the presence of procambium in the globular stage, and vascular tissue in the somatic embryos completely differentiated. The protocol developed allowed us to obtain 10.9 plants per explant.

Key words: abscisic acid, embryogenic callus, Litvay (LVM) medium, Tiger flower



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