Propagation of Ornamental Plants
14(1): 32-39, 2014
SOMATIC EMBRYOGENESIS AND PLANT REGENERATION
IN OSMANTHUS FRAGRANS LOUR.
Jing Jing Zou, Wei Gao, Xuan Cai, Xiang Ling Zeng, and Cai Yun Wang*
Key Laboratory of Horticultural Plant Biology of Ministry of Education, Huazhong Agricultural University, 1 Shizishan str., Hongshan District, Wuhan, Hubei Province, 430070, China, *Fax: + 86 27 8728 2010, *E-mail: email@example.com
A protocol for plant regeneration of sweet osmanthus (Osmanthus fragrans Lour.) via somatic embryogenesis has been developed for the first time. Radicle, hypocotyl, plumule and cotyledon regions of zygotic embryos as explants were optimized at their four developmental stages. They were cultured on Murashige and Skoog (MS) medium with a range of combinations of plant growth regulators, to optimize conditions both for callus initiation and for somatic embryo (SE) development. The highest percentage (88%) of embryogenic callus development occurred with cotyledon segments collected at an early cotyledonary stage when cultured on MS medium with the addition of 1.0 mg l-1 BA and 0.5 mg l-1 2,4-D. The highest percentage (86.7%) of somatic embryogenesis development and SEs per explant (7.5) were obtained on MS medium supplemented with 1.0 mg l-1 BA and 0.5 mg l-1 NAA. Over 50% of mature SEs germinated on MS medium.
Key words: embryogenic callus, plant regeneration, zygotic embryos