Propagation of Ornamental Plants
14(1): 3-12, 2014
IN VITRO PLANT RECOVERY FROM ALGINATE-ENCAPSULATED
CHRYSANTHEMUM × GRANDIFLORUM (RAMAT.) KITAM. SHOOT TIPS
Dariusz Kulus* and MaЕ‚gorzata Zalewska
University of Technology and Life Sciences in Bydgoszcz, Department of Ornamental Plants and Vegetable Crops, Laboratory of Biotechnology, 6 BernardyЕ„ska str., PL-85-029 Bydgoszcz, Poland, *Fax: + 52 37 49505, *E-mail: firstname.lastname@example.org
Shoot tips obtained from bi-weekly in vitro cultures of four cultivars of Chrysanthemum × grandiflorum (Ramat.) Kitam. were encapsulated in 3% calcium alginate hydrogel, containing Murashige and Skoog medium components. Survival and possibilities of further growth were estimated after 7, 30, and 60-day storage on medium with BAP, kinetin or plant growth regulator-free control at room temperature. Afterwards the capsules were transferred to an IAA-enriched medium for another 30 days. Morphogenic response (formation of shoots and roots, as well as their weight and length) of encapsulated buds to variants of the recovery medium was evaluated. The highest recovery rate after 90 days of culture reached 90%. Even though regrowth of shoots was recorded on the control medium, the presence of cytokinins significantly increased its efficiency. BAP stimulated activation of more axillary bud primordia than kinetin, but at the same time inhibited rooting. The obtained results suggest that synthetic seeds technology can be valuable in storage of chrysanthemums.
Key words: caulogenesis, cytokinins, germination, rhizogenesis, synthetic seeds