ISSN 1311-9109 Journal Content





RELATED
CONFERENCES
International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
14(1): 20-25, 2014

PLANT REGENERATION FROM LIQUID CALLUS CULTURES DERIVED
FROM MATURE SEEDS OF PAMPAS GRASS
[CORTADERIA SELLOANA (SCHULT. & SCHULT. F.) ASCH. & GRAEBN.]

Kazuki Shimomae1, Hiroyuki Niimi1, Dong Poh Chin1, Raham Sher Khan1,2, Yuanxue Lu3, Yoichi Ogawa4, and Masahiro Mii1,4*

1Laboratory of Plant Cell Technology, Graduate School of Horticulture, Chiba University, 648 Matsudo, Matsudo, Chiba 271-8510, Japan *Fax +81-47-308-8852,
*E-mail: miim@faculty.chiba-u.jp
2Department of Biotechnology, Abdul Wali Khan University Mardan, Pakistan
3Key Laboratory of Economic Plants and Biotechnology, Kunming Institute of Botany, Chinese Academy of Sciences, 132 Lanhei Road, Kunming, Yunnan, 650204, China
4Center for Environment, Health and Field Sciences, Chiba University, 6-2-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan



An efficient system of plant regeneration from liquid culture of mature seed-derived callus was established in pampas grass [Cortaderia selloana (Schult. & Schult.f.) Asch. & Graebn.]. Calluses were induced from mature seeds on MS medium containing 30 g l-1 maltose, 8 g l-1 agar, and different concentrations (0, 1, 2, and 5 mg l-1) of dicamba, NAA, picloram or 2,4-D. The highest callus induction was obtained on medium containing 5 mg l-1 picloram where 98% explants formed callus. Calluses were subcultured on MS medium containing 2 mg l-1 dicamba, NAA, picloram or 2,4-D and 30 g l-1 maltose for 3 months and the compact calluses obtained were transferred into the same corresponding liquid media. By using 2 month-old liquid-cultured calluses, effects of different concentrations of BAP (0, 0.1, 0.5, and 1 mg l-1) and NAA (0, 0.1, 0.5, and 1 mg l-1) on shoot regeneration were evaluated using MS medium with 30 g l-1 sucrose, 8 g l-1 agar, and 0.5 mg l-1 GA3 supplemented to the basal medium. The highest frequency of shoot formation was obtained in callus induced by picloram on medium containing 0.5 mg l-1 BAP and 0.5 mg l-1 NAA, where 92% of the calluses formed shoots. The calluses maintained high shoot regeneration ability (71-85%) and original ploidy level of diploid during the 8 months of subcultures, although drastic reduction in the regeneration ability (< 23%) and the appearance of tetraploid cells were observed after 12 months of subculture in liquid media.

Key words: micropropagation, ornamental grass, picloram



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