Propagation of Ornamental Plants
12(3): 178-183, 2012
MULTIPLICATION RATES IN LONG TERM CULTURES OF FOUR CULTIVARS OF HEBE
Angelo Gallone1,2, Alan Hunter2, and Gerry Christopher Douglas1*
1Teagasc, Agriculture & Food Development Authority, Kinsealy Research Centre, Malahide Road, Dublin 17, Ireland, *Fax: + 353 18459006, *E-mail: email@example.com
2 School of Agriculture & Food Science, University College Dublin, Dublin 4, Ireland.
Hebe is an ornamental evergreen shrub used for many purposes as a pot plant, garden plant, for foliage and for landscaping. Shoot tips and nodes of four cultivars were used as explants: ‘Wiri Mist’ (Hebe albicans × Hebe diosmifolia), H. albicans ‘Cobb’, ‘Oratia Beauty’, and ‘Nicola’s Blush’. The cultivars were cultured on modified Woody Plant Medium containing 0.2 mg l-1 BA and 0.001 mg l-1 NAA for ‘Wiri Mist’ (M1), 0.2 mg l-1 BA and 0.01 mg l-1 IBA for ‘Cobb’ (M2) and 0.5 mg l-1 BA, 0.01 mg l-1 IBA, and 2.0 mg l-1 GA3, for both ‘Oratia Beauty’ and ‘Nicola’s Blush’ (M3). Shoot cultures free of culturable bacteria were established for all four cultivars. Multiplication rates were recorded over three culture periods at the early phase I and subsequently after a prolonged phase of subculturing, phase II. There was no significant difference in the multiplication rates obtained for phase I compared to the final phase II for each cultivar. The mean multiplication values were 2.7, 3.2, 6.5 and 7.9 in the initial phase I for ‘Cobb’, ‘Wiri Mist’, ‘Nicola’s Blush’ and ‘Oratia Beauty’, respectively and the values for phase II were 3.9, 3.9, 6.0, and 7.9, respectively. This indicates the stability of cultures and the suitability of the media used for all four cultivars.
Key words: bacteria, in vitro, long term multiplication, Plantaginaceae.