Propagation of Ornamental Plants
12(3): 163-170, 2012
SECONDARY SOMATIC EMBRYOGENESIS IN CROCUS VERNUS (L.) HILL
Iyyakkannu Sivanesan1, Moon Sook Son2, Sonali Jana3,
and Byoung Ryong Jeong1,2,3*
1Institute of Agriculture and Life Science, Gyeongsang National University, Jinju, Korea 660-701, *Fax: + 82-55-757-7542, *E-mail: email@example.com
2 Division of Applied Life Science (BK21 Program), Graduate School, Gyeongsang National University, Jinju, Korea 660-701
3 Research Institute of Life Science, Gyeongsang National University, Jinju, Korea 660-701
An efficient procedure for secondary somatic embryogenesis of Crocus vernus is described. The primary somatic embryos were induced from the corm explant of C. vernus on Murashige and Skoog (MS) medium supplemented with 1.0 mg l-1 2,4-D and 0.5 mg l-1 TDZ with a mean number of 52 somatic embryos per explant. The effects of medium formulations (Schenk and Hildebrandt (SH), Gamborg (B-5), Chu (N6), Anderson (AM), and MS and 10 and 45 μmol m-2 s-1 photosynthetic photon flux density were studied for primary somatic embryo induction. The greatest number of somatic embryo induction was obtained on the SH medium amended with 1.0 mg l-1 2,4-D and 0.5 mg l-1 TDZ under 10 μmol m-2 s-1 PPFD. The primary somatic embryos were cultured on SH medium amended with 2iP or BA, in combination with NAA for secondary somatic embryo induction. After 45 days, SH medium fortified with 2.0 mg l-1 BA and 0.5 mg l-1 NAA was found to be the best for secondary embryo induction. Secondary somatic embryos were induced on the surface of globular (88.9%) and heart (95.2%) primary somatic embryos. At 1.0 mg l-1 GA3 92.3% embryo maturation and conversion were observed. The highest frequency (100%) of somatic embryo conversion was obtained on SH medium supplemented with 1.0 mg l-1 GA3 and 6% (w/v) sucrose.
Key words: corm, Dutch crocus, photosynthetic photon flux density, plant regeneration, sucrose, thidiazuron.