ISSN 1311-9109 Journal Content





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International Symposium
on Production and Establishment of Micropropagated Plants
April 19-24, 2015,
Sanremo, Italy


Propagation of Ornamental Plants
12(1): 63-71, 2012

PLANT REGENERATION VIA DIRECT ORGANOGENESIS
OF
XANTHOCERAS SORBIFOLIA BUNGE

Na Zhang1, Jinping Guo1*, Yunxiang Zhang1, and Yuanhuai Han2

1 College of Forestry, Shanxi Agricultural University, Taigu 030801, Shanxi, China,
*Fax: +86 354 6288 693, *E-mail: jinpguo@126.com
2
Institute of Agricultural Bioengineering, College of Agriculture, Shanxi Agricultural University, Taigu 030801, Shanxi, China


Abstract
An efficient and reproducible protocol was developed to induce direct organogenesis from leaf explants of Xanthoceras sorbifolia Bunge. Nine combinations of BA (1.0, 2.0, 3.0 mg l-1) and NAA (0.1, 0.2, 0.5 mg l-1) were conducted to optimize the adventitious shoot induction phase. The results showed that the highest frequency of shoot induction was observed on MS medium supplemented with 2.0 mg l-1 BA and 0.2 mg l-1 NAA at a rate of 96.7%. Higher concentrations of BA resulted in significant decrease in the frequency of shoot induction. The greatest number of shoots was obtained on medium containing 2.0 mg l-1 BA and 0.1 mg l-1 NAA. When in contact with the medium, the abaxial surface had better shoot induction frequency than the adaxial one. During the shoot multiplication phase, several different concentrations of BA (0.1, 0.2, 0.5, 1.0 or 2.0 mg l-1) alone or in combination with 0.1 mg l-1 NAA were studied to determine the optimum multiplication medium. The highest shoot multiplication frequency, mean number of shoots and shoot length were observed on MS medium containing 0.5 mg l-1 BA. Addition of NAA to the medium with different concentrations of BA decreased the multiplication efficiency. During the rooting phase, half-strength MS medium supplemented with different levels of NAA or IBA or IAA (0.2, 0.5, 1.0, 2.0 or 5.0 mg l-1) was utilized to study the rooting potential. The results showed that, the efficiency of rooting on medium containing IBA was significantly higher than with NAA or IAA. The highest frequency of rooting (51.1%) was obtained on half-strength MS medium supplemented with 0.5 mg l-1 IBA. Histological studies showed that the shoot buds had emerged from epidermal cells without callus formation.

Key words: adventitious shoot, histology, leaf explant, plant growth regulator



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