Propagation of Ornamental Plants
11(2): 63-77, 2011
REVIEW OF DOUBLED HAPLOIDY METHODOLOGIES IN ORNAMENTAL SPECIES
Alison M. R. Ferrie* and Karen L. Caswell
Plant Biotechnology Institute - National Research Council, 110 Gymnasium Place, Saskatoon, SK, Canada, *Fax: + 1 306 975-4839,
Ornamental species are an important group of plants produced mainly for their aesthetic value and to enhance both our indoor and outdoor environments. Development of new germplasm with enhanced colours, fragrances, or longevity is an important aspect of plant breeding programs. Tissue culture methods have been utilized to propagate favourable genotypes. Haploidy methodology is commonly used in many agronomically important crops to speed up the breeding program by developing uniform lines. Haploids and doubled haploid protocols can be used for both practical application, as in breeding, mutagenesis, and genetic transformation, as well as basic research (i.e. biochemical, physiological and genomic studies). This review will focus on in vitro haploid production in several ornamental species.
Key words: anther culture, doubled haploid, embryogenesis, gynogenesis, microspore culture, ornamentals