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Propagation of Ornamental Plants
11(1): 40-43, 2011
EFFICIENT IN VITRO PROPAGATION OF CENTAUREA MONTANA L.
Wissam Abou Alaiwi1, Puthiyaparambil Chacko Josekutty2, Stephen Lawrence Goldman1, Shobha Devi Potlakayala2, and Rudrabhatla Venkata Sairam1,2*
1 Plant Science Research Center, University of Toledo, Toledo, Ohio 43606, USA
*Fax: + 1 717-948-6580, *E-mail: svr11@psu.edu
2 School of Science Engineering and Technology, Pennsylvania State University,
777W Harrisburg Pike, Middletown, PA 17057, USA
Abstract
An efficient protocol for in vitro propagation of the flowering ornamentals Centaurea montana was standartized. Adventitious shoots were initiated from leaf explants after 4 weeks of culture on MS medium supplemented with 2% (w/v) sucrose, 0.1 mg l-1 IAA and 0.5 to 2.0 mg l-1 BAP. The best rate of direct shoot proliferation from leaf explants of C. motana (62 ± 15 shoots) was obtained on MS medium supplemented with 0.1 mg l-1 IAA and 2.0 mg l-1 BAP. Elongated shoots, taller than 10 mm were rooted in MS medium containing 0.5 mg l-1 IBA or 1.5 mg l-1 NAA.
Key words: ornamental plant, tissue culture.
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