An efficient adventitious shoot regeneration system was established for Mimulus aurantiacus using internodal explants. The effects of various combinations of NAA (0.0, 0.1 and 0.5 mg l^-1) and TDZ (0.5, 1.0, 2.5 and 5.0 mg l^-1) on adventitious bud induction from leaf and intermodal explants were studied. MS medium supplemented with 0.5 mg l^-1 NAA and 2.5 mg l^-1 TDZ or 0.1 mg l^-1 NAA and 1.0 mg l^-1 TZD was optimal for induction of bud clusters from in vitro grown leaf explants or internodes, respectively. Up to 100% of explants responded with bud cluster formation on these optimal variant of the medium. The presence of NAA in the medium had a major impact on bud cluster induction capacity, whereas when it was omitted from the medium, caulogenic response was significantly lower. The effect of TDZ concentration was less pronounced. Well-established shoot proliferation and elongation was achieved by sub-culturing bud clusters on full-strength MS medium supplemented with 0.1 mg l^-1 IAA and 0.2 mg l^-1 BAP.