The aim of this work was to develop a reliable and most effective method of in vitro production of yellow everlasting (Helichrysum arenarium (L.) Moench). Shoot regeneration was induced by adventitious buds formation on root explants coming from seedlings obtained in vitro. A significant increase of the shoot proliferation rate was observed on yellow everlasting following an application of 1.0 mg l^-1 BA - mean 25.77 shoots per explant. No differences were observed between IAA and NAA as regards their effect on rhizogenesis but the rooting percentage was high even without auxin (94%).