Propagation of Ornamental Plants
9(1): 53-55, 2009
SHOOT REGENERATION FROM SHOOT TIP EXPLANTS OF JUNIPERUS EXCELSA BIEB.
Nikoleta Rifaki1,2, Athanasios Sotirios Economou2,3*,
Stefanos Hatzilazarou2, and Athanasios Hatzistathis1
1School of Forestry and Natural Environment, Aristotle University,
54124 Thessaloniki, Greece,
2School of Agriculture, Aristotle University, 54124 Thessaloniki, Greece,
*Fax: + 30 2310998679, *E-mail: email@example.com
3Institute of Agrobiotechnology, CERTH, 57001 Thermi-Thessaloniki, Greece
The most effective disinfestation treatment for shoot tip explants of field-grown Juniperus exselsa trees was the application of 0.3% HgCl2 for 5 min and 2% NaOCl for 10 min. A variation was observed in the percentages of explant contamination and in the capacity of shoot formation in the six clones tested on WPM. The least contaminated (31%) and the most responsive (56%) explants were those of clone JE-1. The most effective cytokinin in inducing axillary shoot formation on WPM was 2iP at concentrations of 2 and 10 μM (66.7% and 86.7%, respectively), followed by kinetin at concentration of 2 μM (73.3%), while BA failed to induce axillary shoot regeneration as compared to control treatment.
Key words: cytokinins, disinfestation, in vitro culture, Juniperus excelsa, shoot proliferation