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on Production and Establishment of Micropropagated Plants
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Propagation of Ornamental Plants
9(1): 50-52, 2009

ADVENTITIONS SHOOT FORMATION FROM LEAF EXPLANTS OF OLEASTER (ELAEAGNUS ANGUSTIFOLIA L.)

Omid Karami*, Ali Deljou, and Khosro Piri

Department of Biotechnology, Faculty of Agriculture, Bu-Ali Sina University,
Hamadan, Iran *Fax: + 8114227021,
*E-mail: hiva@basu.ac.ir


Abstract
Efficient plant regeneration through adventitious shoot formation was achieved from callus cultures derived from leaf explants of Elaeagnus angustifolia. Calluses were obtained on MS medum containing 3% sucrose and different concentrations of TDZ (0.5, 1, 2, 4, and 6 mg l-1). Maximum percentage response for callus formation was 60% on MS medium supplemented with 2 mg l-1 of TDZ. Adventitious shoot formation was achieved after transfer of calluses to MS medium with 3% sucrose containing different concentrations of BA (0.5, 1, 2, 3, 4, and 6 mg l-1) or kinetin (0.5, 1, 2, 3, 4, and 6 mg l-1). Maximum adventitious shoot formation (44 shoots on 400 mg callus) was observed on MS medium with 2 mg l-1 BA.

Key words: adventitions shoot formation, callus, Elaeagnus angustifolia



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