Propagation of Ornamental Plants
9(1): 39-42, 2009
IN VITRO PROPAGATION OF VIBURNUM DENTATUM L.
VAR. LUCIDUM AITON
Stefanos Hatzilazarou1, Nikoleta Rifaki1, Marianna Patsou1,
Stefanos Kostas1, and Athanasios S. Economou1,2*
1Department of Horticulture, School of Agriculture, Aristotle University, P. O. Box 281, 54124 Thessaloniki, Greece, *Fax: + 30 2310 998679,
2Institute of Agrobiotechnology, CERTH, 57001 Thermi-Thessaloniki, Greece
Appropriate micropropagation regimes were determined for the deciduous species Viburnum dentatum L. var. Lucidum Aiton. Shoot tip explants, collected from greenhouse grown plants and sterilized with 0.1% HgCl2 and 1.2% NaOCl, were established on WPM with 10 μM BA. After two weeks, explants without any signs of contamination were subcultured on WPM, supplemented with BA, 2iP or kinetin at various concentrations (0, 5, 10, 20 μM), for multiple shoot formation. Explants produced more axillary shoots (average 2-4 shoots per explant) with BA than with 2iP or kinetin in a 4-week culture. After subculturing on fresh medium, elongated shoots were excised and induced to root on WPM supplemented with 0, 0.5, 2 or 8 μM IAA, IBA, or NAA. Lower concentrations (0.5 and 2 μM) of auxins were more effective than higher (8 μM) in stimulating rooting, at frequencies higher than 65% with 3-6 roots per shoot. Rooted shoots were transplanted to potting mixture and acclimatized for three weeks by transferring to environmentally controlled conditions. More than 90% of the potted microplants were successfully acclimatized and established in the greenhouse for further growth.
Key words: acclimatization, microplants, micropropagation, root formation, shoot multiplication, Viburnum dentatum