Propagation of Ornamental Plants
9(1): 21-25, 2009
MICROSATELLITE DNA FINGERPRINTING IN MICROPROPAGATED CORNUS MAS L. 'MACROCARPA'
Department of Phytology, Technical University, 24 T.G. Masaryka str.,
960 53 Zvolen, Slovakia, Fax: + 421 45 5332654,
In vitro shoots of Cornus mas 'Macrocarpa' were multiplied by adventitious budding. The resulting micropropagated plants, fully acclimatized to an in vivo environment, did not show any visually detectable morphological variation. Microsatellite fingerprinting in micropropagated plants was evaluated after 4 years of continuous adventitious shoot culture multiplication with the nuclear microsatellite Cf-G17 and two chloroplast microsatellites ccmp2 and ccmp6. Microsatellite primers Cf-G17 produced a band class in a size of 2.4 kb. Primers of the microsatellite ccmp2 gave rise to a band class in a size of 190 bp, whereas microsatellite primers ccmp6 produced a band class in a size of 112 bp. All amplified markers were monomorphic across donor shrub and analysed micropropagated plants, and gave stable fingerprinting patterns.
Key words: Cornelian cherry, genetic stability, microsatellites, tissue culture-derived plantlets