Propagation of Ornamental Plants
8(4): 218-220, 2008
ACID PHOSPHATASE ACTIVITY IN SALT TREATED EMBRYOGENIC SUSPENSION CULTURES OF DACTYLIS GLOMERATA L.
Lyuben Zagorchev1, Christina Hadjiivanova2, and Mariela Odjakova1*
1 Department of Biochemistry, Faculty of Biology, Sofia University, 8 Dragan
Tzankov blvd., 1164 Sofia, Bulgaria, *Fax: +359 2 865 6641,
2 Department of Ecology, University of Forestry, 10 Kliment Ohridski blvd.,
1756 Sofia, Bulgaria
Somatic embryogenesis has been established as an attractive model for exploring the development and differentiation of plants and provides a fast and effective tool for selection of stress-tolerant cultivars. Dactylis glomerata L. is a perennial grass used primarily in mixture with other grasses for grazing, ornamental and soil stabilization purposes. Embryogenic suspension cultures of Dactylis glomerata L. were treated with different salt concentrations - 0.085 M, 0.170 M and 0.255 M NaCl. While 0.085 M NaCl enhances somatic embryogenesis, higher salt concentrations affect negatively regeneration capacity. Acid phosphatases (APases; EC 220.127.116.11) are key enzymes involved in the phosphorus metabolism and are known to be induced by different stresses. APase activity was studied in protein fractions from culture media, cell wall and intracellular space. Different isoforms of APases were identified on electrophoresis with specific staining. The activity is increased in the extracellular fraction of cultures treated with high salt concentrations and is a potential marker for defense responses. There are no significant changes in the intracellular and cell wall fractions.
Key words: acid phosphatases, Dactylis glomerata, orchardgrass, salt stress, somatic embryogenesis